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  • 产品名称：CD40Molecule,TNFReceptorSuperfamilyMember5(CD40)(AA21-193)(Active)protein(AVIta

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD40Molecule,TNFReceptorSuperfamilyMember5(CD40)(AA21-193)(Active)protein(AVItag,Biotin,Histag)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 21.8 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines.
Molecular Weight 21.8 kDa
UniProt P25942
Research Area Surface Receptors of Immune Cells, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Apoptosis/Necrosis, Stem Cells, Cancer, Inflammation
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 21-193) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949014) Biotinylated Human CD40, His Tag on SDS-PAGE under reducing (R) condition. The gel wa...