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  • 产品名称：CD3D&CD3E(Active)protein(FcTag,DYKDDDDKTag,Biotin,Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD3D&CD3E(Active)protein(FcTag,DYKDDDDKTag,Biotin,Histag)
详情介绍：
Brand MABSol®,UltraLys
Specificity The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Characteristics The subunit CD3E carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The subunit CD3D carries a human IgG1 Fc fragment at the C-terminus, followed by a flag tag. The protein has a calculated MW of 39.2 kDa (CD3E), 37.5 kDa (CD3D) . The protein migrates as 40-55 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade Ultra Sensitive
Background T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Molecular Weight 39.2 kDa (CD3E), 37.5 kDa (CD3D)
Comment

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for CD3D & CD3E (Active) protein (Fc Tag,DYKDDDDK Tag,Biotin,His tag) (ABIN2870530) Biotinylated Human CD3E & CD3D on SDS-PAGE under reducing (R) condition. The gel was ...
Binding Studies (Bind) image for CD3D & CD3E (Active) protein (Fc Tag,DYKDDDDK Tag,Biotin,His tag) (ABIN2870530) Immobilized OKT3 mAb at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD3E & CD3D...