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  • 产品名称：FibroblastGrowthFactor2(Basic)(FGF2)(AA143-288)(Active)protein(Biotin)

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  • 产品厂商：ACROBiosystems
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简单介绍：
FibroblastGrowthFactor2(Basic)(FGF2)(AA143-288)(Active)protein(Biotin)
详情介绍：
Brand MABSol®,UltraLys
Specificity The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Characteristics The product does not contain any epitope tags. The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa on a SDS-PAGE gel under reducing (R) condition.
Purity >95 % as determined by reduced SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade Ultra Sensitive
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human FGFR4 /CD334 Protein (Catalog # FG4-H5228) can bind Biotinylated rh-bFGF (Catalog # BFF-H8117).

SDS-PAGE: The primary amine of Biotinylated rhbFGF was labeled by biotin for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
Background FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60 % amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. bFGF is a critical component of human embryonic stem cell culture medium. bFGF is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.
Molecular Weight 16.5 kDa
NCBI Accession NP_001997
UniProt P09038
Research Area Organogenesis, Angiogenesis, Growth Factors
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus
Comment

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) (Active) protein (Biotin) (ABIN2180651) Biotinylated Human FGF basic on SDS-PAGE under reducing (R) condition. The gel was st...