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  • 产品名称：GrowthFactor,AugmenterofLiverRegeneration(GFER)(AA81-205)(Active)Protein

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  • 产品厂商：ACROBiosystems
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简单介绍：
GrowthFactor,AugmenterofLiverRegeneration(GFER)(AA81-205)(Active)Protein
详情介绍：
Brand ActiveMax®
Characteristics This protein carries no "tag". The protein has a calculated MW of 15.2 kDa. The protein migrates as 16 kDa under reducing (R) condition (SDS-PAGE).
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Growth factor, augmenter of liver regeneration (GFER) is also known as FAD-linked sulfhydryl oxidase ALR, which belongs to the Erv1/ALR family of proteins. This family can be found in higher and lower eukaryotes. There are two isoform of GFER: Isoform 1 and isoform 2. Isoform 2 missing 1 - 80 aa. Isoform 1 is mainly located in mitochondrion intermembrane space, while Isoform 1 is secreted to cytoplasm. Isoform 1 of GFER regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. The isoform 2 of GFER may act as an autocrine hepatotrophic growth factor promoting liver regeneration.
Molecular Weight 15.2 kDa
NCBI Accession NP_005253
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Growth Factor, Augmenter of Liver Regeneration (GFER) (AA 81-205) (Active) Protein (ABIN2181149) Growth Factor, Augmenter of Liver Regeneration (GFER) (AA 81-205) (Active) protein
Background publications Sztolsztener, Brewinska, Guiard, Chacinska: "Disulfide bond formation: sulfhydryl oxidase ALR controls mitochondrial biogenesis of human MIA40." in: Traffic (Copenhagen, Denmark), Vol. 14, Issue 3, pp. 309-20, 2013 (PubMed).

Daithankar, Farrell, Thorpe: "Augmenter of liver regeneration: substrate specificity of a flavin-dependent oxidoreductase from the mitochondrial intermembrane space." in: Biochemistry, Vol. 48, Issue 22, pp. 4828-37, 2009 (PubMed).

Di Fonzo, Ronchi, Lodi, Fassone, Tigano, Lamperti, Corti, Bordoni, Fortunato, Nizzardo, Napoli, Donadoni, Salani, Saladino, Moggio, Bresolin, Ferrero, Comi: "The mitochondrial disulfide relay system protein GFER is mutated in autosomal-recessive myopathy with cataract and combined respiratory-chain deficiency." in: American journal of human genetics, Vol. 84, Issue 5, pp. 594-604, 2009 (PubMed).