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  • 产品名称：FcFragmentofIgG,Receptor,Transporter,alpha(FCGRT)(AA21-119)(Active)protein(Stre

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  • 产品厂商：ACROBiosystems
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简单介绍：
FcFragmentofIgG,Receptor,Transporter,alpha(FCGRT)(AA21-119)(Active)protein(StrepTag,Histag)
详情介绍：
Brand MABSol®
Characteristics Human FcRn / FCGRT & B2M heterodimer protein, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa FCGRT and 13.1 kDa (B2M). Subunit FCGRT is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The predicted N-terminus is Ala 24(FCGRT) & Ile 21(B2M). The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind human IgG1 in a functional ELISA. Immobilized rh FcRN & B2M (Catalog # FCM-H5286) at 2 μg/mL (100 μL/well ) can bind human IgG1 with a linear ranger of 0.05-2 μg/mL. Measured by its binding ability to human IgG1 in a SPR assay (Biacore 2000). Immobilized human FcRN&B2M can bind human IgG1 with affinity constant of uM range.
Sub Type Fusionprotein
Background FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
Molecular Weight 31.2 kDa (FCGRT), 13.1 kDa (B2M)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Fc Fragment of IgG, Receptor, Transporter, alpha (FCGRT) (AA 21-119) (Active) protein (Strep Tag,His tag) (ABIN2181085) Human FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The gel was staine...
Binding Studies (Bind) image for Fc Fragment of IgG, Receptor, Transporter, alpha (FCGRT) (AA 21-119) (Active) protein (Strep Tag,His tag) (ABIN2181085) Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Human F...
Background publications Goebl, Babbey, Datta-Mannan, Witcher, Wroblewski, Dunn: "Neonatal Fc receptor mediates internalization of Fc in transfected human endothelial cells." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5490-505, 2008 (PubMed).

Gorevic, Munoz, Casey, DiRaimondo, Stone, Prelli, Rodrigues, Poulik, Frangione: "Polymerization of intact beta 2-microglobulin in tissue causes amyloidosis in patients on chronic hemodialysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 20, pp. 7908-12, 1986 (PubMed).