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  • 产品名称：Fms-RelatedtyrosineKinase3Ligand(FLT3LG)(AA27-185)(Active)Protein

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  • 产品厂商：ACROBiosystems
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简单介绍：
Fms-RelatedtyrosineKinase3Ligand(FLT3LG)(AA27-185)(Active)Protein
详情介绍：
Brand ActiveMax®
Characteristics This protein carries no "tag". The protein has a calculated MW of 18.9 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of human acute myeloid leukemia cell line OCI-AML5. The ED50 < 0.5 ng/mL, corresponding to a specific activity of >2X106 Unit/mg

SDS-PAGE: resulting from glycosylation.
Background FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.
Molecular Weight 18.9 kDa
UniProt P49771
Pathways RTK Signaling
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-185) (Active) Protein (ABIN2181107) Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-185) (Active) protein
Background publications Kalberer, Siegler, Wodnar-Filipowicz: "Human NK cell development in NOD/SCID mice receiving grafts of cord blood CD34+ cells." in: Blood, Vol. 102, Issue 1, pp. 127-35, 2003 (PubMed).

Wodnar-Filipowicz: "Flt3 ligand: role in control of hematopoietic and immune functions of the bone marrow." in: News in physiological sciences : an international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society, Vol. 18, pp. 247-51, 2003 (PubMed).