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  • 产品名称：ProteinL(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ProteinL(Active)protein(Histag)
详情介绍：
Brand MABSol®
Characteristics Recombinant Protein L Protein is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 36-38 kDa polypeptide .Protein L Protein can bind to VL-Kappa.
Purity >98 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
Gene ID 17
Research Area Bacteria, Gram positive bacteria
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 100 mM NaH2PO4, pH 8.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Protein L (Active) protein (His tag) (ABIN2181671) Protein L (Active) protein (His tag)
Background publications Tesselaar, Xiao, Arens, van Schijndel, Schuurhuis, Mebius, Borst, van Lier: "Expression of the murine CD27 ligand CD70 in vitro and in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 33-40, 2002 (PubMed).

Tesselaar, Xiao, Arens, van Schijndel, Schuurhuis, Mebius, Borst, van Lier: "Expression of the murine CD27 ligand CD70 in vitro and in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 33-40, 2002 (PubMed).

De Château, Nilson, Erntell, Myhre, Magnusson, Akerström, Björck: "On the interaction between protein L and immunoglobulins of various mammalian species." in: Scandinavian journal of immunology, Vol. 37, Issue 4, pp. 399-405, 1993 (PubMed).

De Château, Nilson, Erntell, Myhre, Magnusson, Akerström, Björck: "On the interaction between protein L and immunoglobulins of various mammalian species." in: Scandinavian journal of immunology, Vol. 37, Issue 4, pp. 399-405, 1993 (PubMed).

Björck: "Protein L. A novel bacterial cell wall protein with affinity for Ig L chains." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 4, pp. 1194-7, 1988 (PubMed).