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  • 产品名称：serpinPeptidaseInhibitor,CladeE(Nexin,PlasminogenActivatorInhibitorType1),Membe

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  • 产品厂商：ACROBiosystems
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简单介绍：
serpinPeptidaseInhibitor,CladeE(Nexin,PlasminogenActivatorInhibitorType1),Member2(SERPINE2)(AA20-397)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 42.7 kDa. The protein migrates as 45-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK(Dnp) - NH2.

SDS-PAGE: due to glycosylation.
Background SERPINE2 is also known as Glia-derived nexin (GDN), Peptidase inhibitor 7 (PI7), Protease nexin 1(PN1). SERPINE2 is a secreted glycoprotein which belongs to the serpin family. SerpinE1 is the primary physiological inhibitor of the two plasminogen activators urokinase (uPA) and tissue plasminogen activator (tPA). PAI-1 / GDN is also implicated in adipose tissue development. It suggests that PAI-1 inhibitors serve in the control of atherothrombosis. Defects in Serpin E1 / PN1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to SerpinE1 defect in the plasma.
Molecular Weight 42.7 kDa
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 20 mM Sodium Acetate, 100 mM NaCl, pH 6.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 2 (SERPINE2) (AA 20-397) (Active) protein (His tag) (ABIN2181749) Human Serpin E2, His Tag on SDS-PAGE under reducing (R) condition. The gel was staine...
Background publications Gloor, Odink, Guenther, Nick, Monard: "A glia-derived neurite promoting factor with protease inhibitory activity belongs to the protease nexins." in: Cell, Vol. 47, Issue 5, pp. 687-93, 1987 (PubMed).

Scott, Bergman, Bajpai, Hersh, Rodriguez, Jones, Barreda, Watts, Baker: "Protease nexin. Properties and a modified purification procedure." in: The Journal of biological chemistry, Vol. 260, Issue 11, pp. 7029-34, 1985 (PubMed).