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  • 产品名称：ADAMMetallopeptidaseDomain8(ADAM8)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ADAMMetallopeptidaseDomain8(ADAM8)(Active)protein(Histag)
详情介绍：
Characteristics rh ADAM8 fused with a polyhistidine tag at the C-terminus, has a calculated MW of 71.6 kDa, which migrates as a 120-150 kDa band in DTT-reduced SDS-PAGE due to glycosylation. Two main fragments bands are visible with apparent MW of 67 kDa and 46 kDa respectively in reduced SDS-PAGE as a result of its being prone to proteolytic cleavage.
Purity >85 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Disintegrin and metalloproteinase domain-containing protein 8 is also known as ADAM8, Cell surface antigen MS2 and CD antigen CD156a, which belongs to the ADAM (a disintegrin and metalloprotease domain) family. ADAM family play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. ADAM8 / CD156a contains 1 disintegrin domain, 1 EGF-like domain and 1 peptidase M12B domain. ADAM8 / MS2 / CD156a is expressed on neutrophils and monocytes. ADAM8 / MS2 possible involvement in extravasation of leukocytes.
Molecular Weight 71.6 kDa
UniProt P78325
Research Area Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix, Apoptosis/Necrosis
Pathways Activation of Innate immune Response, M Phase
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, 20 mM CaCl2, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for ADAM Metallopeptidase Domain 8 (ADAM8) (Active) protein (His tag) (ABIN2180563) Human ADAM8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Hall, Shieh, Day, Caspers, Chrencik, Williams, Pegg, Pauley, Moon, Krahn, Fischer, Kiefer, Tomasselli, Zack: "Structure of human ADAM-8 catalytic domain complexed with batimastat." in: Acta crystallographica. Section F, Structural biology and crystallization communications, Vol. 68, Issue Pt 6, pp. 616-21, 2012 (PubMed).

Yoshiyama, Higuchi, Kataoka, Matsuura, Yamamoto: "CD156 (human ADAM8): expression, primary amino acid sequence, and gene location." in: Genomics, Vol. 41, Issue 1, pp. 56-62, 1997 (PubMed).