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  • 产品名称：Glycoprotein/GP(Virus)(AA33-304)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Glycoprotein/GP(Virus)(AA33-304)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 31.8 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160- kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
Molecular Weight 31.8 kDa
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Glycoprotein / GP (Virus) (AA 33-304) (Active) protein (His tag) (ABIN2181010) Ebolavirus (subtype Bundibugyo,strain Uganda 2007) GP1 on SDS-PAGE under reducing (R)...
Background publications Ali, Saeed, Ali, Haidar: "Determinants of helmet use behaviour among employed motorcycle riders in Yazd, Iran based on theory of planned behaviour." in: Injury, Vol. 42, Issue 9, pp. 864-9, 2011 (PubMed).

Marzi, Akhavan, Simmons, Gramberg, Hofmann, Bates, Lingappa, Pöhlmann: "The signal peptide of the ebolavirus glycoprotein influences interaction with the cellular lectins DC-SIGN and DC-SIGNR." in: Journal of virology, Vol. 80, Issue 13, pp. 6305-17, 2006 (PubMed).

Chandran, Sullivan, Felbor, Whelan, Cunningham: "Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection." in: Science (New York, N.Y.), Vol. 308, Issue 5728, pp. 1643-5, 2005 (PubMed).