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  • 产品名称：Endosulfinealpha(ENSA)(AA2-121)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Endosulfinealpha(ENSA)(AA2-121)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.2 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Alpha-endosulfine (ENSA),a member of the endosulfine family, is also known as ARPP-19e,which is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. ENSA is a protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, ENSA is able to specifically interact with PPP2R2D (PR55-delta) and inhibit its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). ENSA acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
Molecular Weight 14.2 kDa
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Endosulfine alpha (ENSA) (AA 2-121) (Active) protein (His tag) (ABIN2181008) Human Alpha-endosulfine, His Tag on SDS-PAGE under reducing (R) condition. The gel wa...
Background publications Boettcher, Hartman, Ladror, Qi, Woods, George, Rienstra: "(1)H, (13)C, and (15)N resonance assignment of the cAMP-regulated phosphoprotein endosulfine-alpha in free and micelle-bound states." in: Biomolecular NMR assignments, Vol. 1, Issue 2, pp. 167-9, 2009 (PubMed).

Woods, Boettcher, Zhou, Kloepper, Hartman, Ladror, Qi, Rienstra, George: "Conformation-specific binding of alpha-synuclein to novel protein partners detected by phage display and NMR spectroscopy." in: The Journal of biological chemistry, Vol. 282, Issue 47, pp. 34555-67, 2007 (PubMed).

Héron, Virsolvy, Apiou, Le Cam, Bataille: "Isolation, characterization, and chromosomal localization of the human ENSA gene that encodes alpha-endosulfine, a regulator of beta-cell K(ATP) channels." in: Diabetes, Vol. 48, Issue 9, pp. 1873-6, 1999 (PubMed).