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  • 产品名称：SpermAdhesionMolecule1(PH-20Hyaluronidase,ZonaPellucidaBinding)(SPAM1)(AA36-482

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  • 产品厂商：ACROBiosystems
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简单介绍：
SpermAdhesionMolecule1(PH-20Hyaluronidase,ZonaPellucidaBinding)(SPAM1)(AA36-482)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 52.1 kDa. The protein migrates as 64-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N - acetyl - beta - D - glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Molecular Weight 52.1 kDa
UniProt P38567
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM NaCl, pH 7.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 36-482) (Active) protein (His tag) (ABIN2181785) Human PH20, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Arming, Strobl, Wechselberger, Kreil: "In vitro mutagenesis of PH-20 hyaluronidase from human sperm." in: European journal of biochemistry / FEBS, Vol. 247, Issue 3, pp. 810-4, 1997 (PubMed).

Gmachl, Sagan, Ketter, Kreil: "The human sperm protein PH-20 has hyaluronidase activity." in: FEBS letters, Vol. 336, Issue 3, pp. 545-8, 1994 (PubMed).

Lin, Kimmel, Myles, Primakoff: "Molecular cloning of the human and monkey sperm surface protein PH-20." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 21, pp. 10071-5, 1993 (PubMed).