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  • 产品名称：ColonyStimulatingFactor3(Granulocyte)(CSF3)(AA31-204)(Active)Protein

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  • 产品厂商：ACROBiosystems
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简单介绍：
ColonyStimulatingFactor3(Granulocyte)(CSF3)(AA31-204)(Active)Protein
详情介绍：
Brand ActiveMax®
Characteristics This protein carries no "tag". The protein has a calculated MW of 18.7 kDa. The protein migrates as 18-21 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of M-NFS-60 cells. The ED50<0.05ng ml,="" corresponding="" to="" a="" specific="" activity="" of="">2X107 Unit/mg.

SDS-PAGE: of reduced rhG-CSF.
Background Granulocyte colony-stimulating factor (G-CSF or GCSF) is also known as colony-stimulating factor 3, CSF3, C17orf33, CSF3OS, GCSF, MGC45931. It is a glycoprotein, growth factor and cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF regulates them using Janus kinase (JAK)/signal transducer and activator of transcription (STAT) and Ras /mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway. G-CSF is produced by endothelium, macrophages, and a number of other immune cells. The natural human glycoprotein exists in two forms, a 174- and 180-amino-acid-long protein of molecular weight 19,600 grams per mole. G-CSF can effect on the hematopoietic system and neuronal cells as a neurotrophic factor. The action of G-CSF in the central nervous system is to induce neurogenesis, to increase the neuroplasticity and to counteract apoptosis. G-CSF stimulates the production of white blood cells (WBC). In oncology and hematology, a recombinant form of G-CSF is used with certain cancer patients to accelerate recovery from neutropenia after chemotherapy, allowing higher-intensity treatment regimens. Another form of recombinant human G-CSF called lenograstim is synthesised in Chinese Hamster Ovary cells (CHO cells). The recombinant human G-CSF synthesised in an E. coli expression system is called filgrastim.
Molecular Weight 18.7 kDa
NCBI Accession NP_757373
UniProt P09919
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) Protein (ABIN2181135) Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) protein
Background publications Pitzer, Krüger, Plaas, Kirsch, Dittgen, Müller, Laage, Kastner, Suess, Spoelgen, Henriques, Ehrenreich, Schäbitz, Bach, Schneider: "Granulocyte-colony stimulating factor improves outcome in a mouse model of amyotrophic lateral sclerosis." in: Brain : a journal of neurology, Vol. 131, Issue Pt 12, pp. 3335-47, 2008 (PubMed).

Schneider, Krüger, Steigleder, Weber, Pitzer, Laage, Aronowski, Maurer, Gassler, Mier, Hasselblatt, Kollmar, Schwab, Sommer, Bach, Kuhn, Schäbitz: "The hematopoietic factor G-CSF is a neuronal ligand that counteracts programmed cell death and drives neurogenesis." in: The Journal of clinical investigation, Vol. 115, Issue 8, pp. 2083-98, 2005 (PubMed).