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  • 产品名称：FcFragmentofIgG,LowAffinityIIIa,Receptor(CD16a)(FCGR3A)(AA17-208)(Active)protei

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  • 产品厂商：ACROBiosystems
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简单介绍：
FcFragmentofIgG,LowAffinityIIIa,Receptor(CD16a)(FCGR3A)(AA17-208)(Active)protein(Histag)
详情介绍：
Brand MABSol®
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 22.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind human IgG with an estimated KD < 45 nM.

SDS-PAGE: due to glycosylation.
Background CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
Molecular Weight 22.7 kDa
UniProt P08637
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-208) (Active) protein (His tag) (ABIN2181078) Human FCGR3A / CD16a (F158), His Tag on SDS-PAGE under reducing (R) condition. The ge...
Binding Studies (Bind) image for Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-208) (Active) protein (His tag) (ABIN2181078) Immobilized Yervoy Ipilimumab at 5 μg/mL (100 μl/well) can bind Human FCGR3A / CD16a ...
Background publications Webster, Kedzierska, Azzam, Paukovics, Wilson, Crowe, Jaworowski: "Phagocytosis stimulates mobilization and shedding of intracellular CD16A in human monocytes and macrophages: inhibition by HIV-1 infection." in: Journal of leukocyte biology, Vol. 79, Issue 2, pp. 294-302, 2006 (PubMed).

Nagarajan, Chesla, Cobern, Anderson, Zhu, Selvaraj: "Ligand binding and phagocytosis by CD16 (Fc gamma receptor III) isoforms. Phagocytic signaling by associated zeta and gamma subunits in Chinese hamster ovary cells." in: The Journal of biological chemistry, Vol. 270, Issue 43, pp. 25762-70, 1995 (PubMed).