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  • 产品名称：R-Spondin1(RSPO1)(AA21-146)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
R-Spondin1(RSPO1)(AA21-146)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.7 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/mL (100 μL/well) can bind human LIMPII with a linear ranger of 12.5 - 1000 ng/mL.

SDS-PAGE: under reducing conditions, the apparent molecular mass of rhRSPO1/146 is approximately 20-25 kDa due to the glycosylation.
Background R-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus. Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively. R-Spondin-1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. Human RSPO1 disruption results in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. It has been shown that the complete female-to-male sex reversal is due to the absence of the testis-determining gene, SRY. R-Spondin-1 regulates Wnt/β-catenin by competing with the Wnt antagonist DKK1 for binding to the Wnt co receptors, Kremen and LRP6, reducing their DKK1 mediated internalization. Reports differ on whether R-spondin 1 binds LRP6 directly.
Molecular Weight 16.7 kDa
UniProt Q2MKA7
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for R-Spondin 1 (RSPO1) (AA 21-146) (Active) protein (His tag) (ABIN2181686) Human R-Spondin 1 (21-146), His Tag on SDS-PAGE under reducing (R) condition. The gel...
Background publications Binnerts, Kim, Bright, Patel, Tran, Zhou, Leung, Liu, Lomas, Dixon, Hazell, Wagle, Nie, Tomasevic, Williams, Zhan, Levy, Funk, Abo: "R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 37, pp. 14700-5, 2007 (PubMed).

Wei, Yokota, Semenov, Doble, Woodgett, He: "R-spondin1 is a high affinity ligand for LRP6 and induces LRP6 phosphorylation and beta-catenin signaling." in: The Journal of biological chemistry, Vol. 282, Issue 21, pp. 15903-11, 2007 (PubMed).

Nam, Turcotte, Yoon: "Dynamic expression of R-spondin family genes in mouse development." in: Gene expression patterns : GEP, Vol. 7, Issue 3, pp. 306-12, 2006 (PubMed).

Kazanskaya, Glinka, del Barco Barrantes, Stannek, Niehrs, Wu: "R-Spondin2 is a secreted activator of Wnt/beta-catenin signaling and is required for Xenopus myogenesis." in: Developmental cell, Vol. 7, Issue 4, pp. 525-34, 2004 (PubMed).