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  • 产品名称：R-Spondin1(RSPO1)(AA21-263)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
R-Spondin1(RSPO1)(AA21-263)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.6 kDa. The protein migrates as 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized human RSPO1 at 20 μg/mL ( 100 μL/well ) can bind human LIMPII with a linear ranger of 31.25 - 1000 ng/mL.

SDS-PAGE: under reducing conditions, the apparent molecular mass of rhRSPO1 is approximately 40 kDa due to the glycosylation.
Background R-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), which is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus.
Molecular Weight 27.6 kDa
UniProt Q2MKA7
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for R-Spondin 1 (RSPO1) (AA 21-263) (Active) protein (His tag) (ABIN2181684) Human R-Spondin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications Wei, Yokota, Semenov, Doble, Woodgett, He: "R-spondin1 is a high affinity ligand for LRP6 and induces LRP6 phosphorylation and beta-catenin signaling." in: The Journal of biological chemistry, Vol. 282, Issue 21, pp. 15903-11, 2007 (PubMed).

Binnerts, Kim, Bright, Patel, Tran, Zhou, Leung, Liu, Lomas, Dixon, Hazell, Wagle, Nie, Tomasevic, Williams, Zhan, Levy, Funk, Abo: "R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 37, pp. 14700-5, 2007 (PubMed).

Nam, Turcotte, Yoon: "Dynamic expression of R-spondin family genes in mouse development." in: Gene expression patterns : GEP, Vol. 7, Issue 3, pp. 306-12, 2006 (PubMed).

Kazanskaya, Glinka, del Barco Barrantes, Stannek, Niehrs, Wu: "R-Spondin2 is a secreted activator of Wnt/beta-catenin signaling and is required for Xenopus myogenesis." in: Developmental cell, Vol. 7, Issue 4, pp. 525-34, 2004 (PubMed).