产品详情
查看大图

  • 产品名称：Kallikrein8(KLK8)(AA29-260)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Kallikrein8(KLK8)(AA29-260)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.8 kDa. The protein migrates as 38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit thrombin (R&D Systems, Catalog# 1473SE) cleavage of a fluorogenic peptide substrate Boc-VPR-AMC ( R&D Systems , Catalog# ES011). The specific activity is >500 pmoles / min / μg.

SDS-PAGE: due to glycosylation.
Background Kallikrein-8 (KLK8) is also known as Neuropsin (NP or NRPN), Ovasin, Serine protease 19 (PRSS19), Tumor-associated differentially expressed gene 14 protein (TADG-14), which belongs to the peptidase S1 family and Kallikrein subfamily. KLK8 contains 1 peptidase S1 domain. KLK8 is pH dependence protein and the optimum pH is 8.5, and the protein is active from pH 7-10. KLK8 is expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer. KLK8 cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys, and the catalytic activity of KLK8 is inhibited by a range of serine protease inhibitors including antipain, aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor.
Molecular Weight 25.8 kDa
UniProt O60259
Pathways Complement System
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 8.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Kallikrein 8 (KLK8) (AA 29-260) (Active) protein (His tag) (ABIN2181444) Human Kallikrein 8, His Tag on SDS-PAGE under reducing (R) condition. The gel was sta...
Background publications Brännström, Sellin, Holmfeldt, Brattsand, Gullberg: "The Schistosoma mansoni protein Sm16/SmSLP/SmSPO-1 assembles into a nine-subunit oligomer with potential To inhibit Toll-like receptor signaling." in: Infection and immunity, Vol. 77, Issue 3, pp. 1144-54, 2009 (PubMed).

Brattsand, Stefansson, Hubiche, Nilsson, Egelrud: "SPINK9: a selective, skin-specific Kazal-type serine protease inhibitor." in: The Journal of investigative dermatology, Vol. 129, Issue 7, pp. 1656-65, 2009 (PubMed).

Scott, Sun, Whisstock, Kato, Bird: "SerpinB6 is an inhibitor of kallikrein-8 in keratinocytes." in: Journal of biochemistry, Vol. 142, Issue 4, pp. 435-42, 2007 (PubMed).

Rajapakse, Ogiwara, Takano, Moriyama, Takahashi: "Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins." in: FEBS letters, Vol. 579, Issue 30, pp. 6879-84, 2005 (PubMed).