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  • 产品名称：TCellImmunoreceptorwithIgandITIMDomains(TIGIT)(AA22-141)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
TCellImmunoreceptorwithIgandITIMDomains(TIGIT)(AA22-141)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 39.7 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Molecular Weight 39.7 kDa
UniProt Q495A1
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) (Active) protein (Fc Tag) (ABIN2181816) Human TIGIT, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
image for T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) (Active) protein (Fc Tag) (ABIN2181816) Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Ta...
Background publications Stengel, Harden-Bowles, Yu, Rouge, Yin, Comps-Agrar, Wiesmann, Bazan, Eaton, Grogan: "Structure of TIGIT immunoreceptor bound to poliovirus receptor reveals a cell-cell adhesion and signaling mechanism that requires cis-trans receptor clustering." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 14, pp. 5399-404, 2012 (PubMed).

Lozano, Dominguez-Villar, Kuchroo, Hafler: "The TIGIT/CD226 axis regulates human T cell function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 8, pp. 3869-75, 2012 (PubMed).

Yu, Harden, Gonzalez, Francesco, Chiang, Irving, Tom, Ivelja, Refino, Clark, Eaton, Grogan: "The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells." in: Nature immunology, Vol. 10, Issue 1, pp. 48-57, 2008 (PubMed).