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  • 产品名称：V-AktMurineThymomaViralOncogeneHomolog1(AKT1)(AA1-480)(Active)protein(StrepTag,

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  • 产品厂商：ACROBiosystems
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简单介绍：
V-AktMurineThymomaViralOncogeneHomolog1(AKT1)(AA1-480)(Active)protein(StrepTag,Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus, followed by a twin strep tag. The protein has a calculated MW of 59.5 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by reduced SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background RAC-alpha serine/threonine-protein kinase (AKT1) is also known PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt and RAC-PK-alpha, which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and RAC subfamily and is expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase).
Molecular Weight 59.5 kDa
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 20 mM Tris, 150 mM NaCl, pH 8.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) (Active) protein (Strep Tag,His tag) (ABIN2180576) Human Akt1, His Tag & Strep Tag on SDS-PAGE under reducing (R) condition. The gel was...
Background publications Goshima, Kawamura, Fukumoto, Miura, Honma, Satoh, Wakamatsu, Yamamoto, Kimura, Nishikawa, Andoh, Iida, Ishikawa, Ito, Kagawa, Kaminaga, Kanehori, Kawakami, Kenmochi, Kimura, Kobayashi, Kuroita et al.: "Human protein factory for converting the transcriptome into an in vitro-expressed proteome,. ..." in: Nature methods, Vol. 5, Issue 12, pp. 1011-7, 2008 (PubMed).

Delcommenne, Tan, Gray, Rue, Woodgett, Dedhar: "Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 19, pp. 11211-6, 1998 (PubMed).

Youngson, Jones: "Management of the impalpable testis: long-term results of the preperitoneal approach." in: Journal of pediatric surgery, Vol. 26, Issue 5, pp. 618-20, 1991 (PubMed).

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991 (PubMed).