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  • 产品名称：VascularEndothelialGrowthFactor(VEGF)(AA27-147)(Active)Protein

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  • 产品厂商：ACROBiosystems
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简单介绍：
VascularEndothelialGrowthFactor(VEGF)(AA27-147)(Active)Protein
详情介绍：
Brand ActiveMax®
Characteristics This protein carries no "tag". The protein has a calculated MW of 14 kDa (monomer). As a result of glycosylation, the protein migrates as 19 kDa and 20 kDa under reducing (R) condition, and 40-45 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of HUVEC cells. The ED50<1ng ml,="" corresponding="" to="" a="" specific="" activity="" of="">1X106 Unit/mg.

SDS-PAGE: of reduced (+) and non-reduced (-) rhVEGF121.
Background Vascular endothelial growth factor (VEGF) is also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87 % aa sequence identity with corresponding regions of mouse and rat, 93 % with feline, equine and bovine, and 91 %, 95 % and 96 % with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.
Molecular Weight 14 kDa (monomer)
NCBI Accession NP_001020541
UniProt P15692
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Vascular Endothelial Growth Factor (VEGF) (AA 27-147) (Active) Protein (ABIN2181899) Human VEGF121 on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel...
Binding Studies (Bind) image for Vascular Endothelial Growth Factor (VEGF) (AA 27-147) (Active) Protein (ABIN2181899) Immobilized Human VEGF121 with a linear range of 0.15-2.5 μg/mL.
Product cited in: Stafford, Matsumoto, Yin, Cai, Fung, Stephenson, Gill, You, Lin, Wang, Masikat, Li, Penta, Steiner, Baliga, Murray, Thanos, Hallam, Sato: "In vitro Fab display: a cell-free system for IgG discovery." in: Protein engineering, design & selection : PEDS, Vol. 27, Issue 4, pp. 97-109, 2014 (PubMed).

Background publications Barr, Byrne, Duffy, Condron, Devocelle, Harriott, Bouchier-Hayes, Harmey: "A peptide corresponding to the neuropilin-1- on VEGF(165) induces apoptosis of neuropilin-1-expressing breast tumour cells." in: British journal of cancer, Vol. 92, Issue 2, pp. 328-33, 2005 (PubMed).

Robinson, Stringer: "The splice variants of vascular endothelial growth factor (VEGF) and their receptors." in: Journal of cell science, Vol. 114, Issue Pt 5, pp. 853-65, 2001 (PubMed).