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  • 产品名称：CD86Molecule(CD86)(AA26-245)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD86Molecule(CD86)(AA26-245)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.5 kDa. As a result of glycosylation, the protein migrates as 66-100 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells.The ED50 for this effect is typically 0.04 - 0.2 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized rhB7-2 / CD86 Fc Chimera (Catalog # CD6-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated rh CTLA4, His.Avi Tag (Catalog # CT4-H82E4) with a linear range of 0.1 - 1 μg/mL.

SDS-PAGE: due to glycosylation.
Background Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.
Molecular Weight 51.5 kDa
UniProt P42081
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD86 Molecule (CD86) (AA 26-245) (Active) protein (Fc Tag) (ABIN2180621) Human B7-2, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. Th...
Binding Studies (Bind) image for CD86 Molecule (CD86) (AA 26-245) (Active) protein (Fc Tag) (ABIN2180621) Immobilized Human B7-2, Fc Tag with a linear range of 1-20 ng/mL.
Binding Studies (Bind) image for CD86 Molecule (CD86) (AA 26-245) (Active) protein (Fc Tag) (ABIN2180621) Immobilized Human B7-2, Fc Tag with a linear range of 0.1 - 1 μg/mL.
Background publications Yadav, Sarvetnick: "B7-2 regulates survival, phenotype, and function of APCs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 10, pp. 6236-41, 2007 (PubMed).

Chen, Gault, Shen, Nabavi: "Molecular cloning and expression of early T cell costimulatory molecule-1 and its characterization as B7-2 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4929-36, 1994 (PubMed).