产品详情
查看大图

  • 产品名称：C-TypeLectinDomainFamily3,MemberB(CLEC3B)(AA22-202)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
C-TypeLectinDomainFamily3,MemberB(CLEC3B)(AA22-202)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 0.1 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF (ACRObiosystems, Catalog # HGF-H4226) at 5 μg/mL (100 μL/well), the concentration of recombinant human CLEC3B that produces 50% of the optimal binding response is found to be approximately 1 - 10 μg/mL.

SDS-PAGE: due to glycosylation.
Background C-type lectin domain family 3 member B (CLEC3B) is also known as Tetranectin, Plasminogen kringle 4-binding protein, is a secreted homotrimer. CLEC3B contains 1 C-type lectin domain and mainly found in plasma. Tetranectin / CLEC3B binds to plasminogen and to isolated kringle 4. CLEC3B may be involved in the packaging of molecules destined for exocytosis. Decreased plasma Tetranectin or increased Tetranectin in stroma of cancers correlates with cancer progression and adverse prognosis.
Molecular Weight 21 kDa
UniProt P05452
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for C-Type Lectin Domain Family 3, Member B (CLEC3B) (AA 22-202) (Active) protein (His tag) (ABIN2180883) Human CLEC3B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained o...
Background publications Jaquinod, Holtet, Etzerodt, Clemmensen, Thøgersen, Roepstorff: "Mass spectrometric characterisation of post-translational modification and genetic variation in human tetranectin." in: Biological chemistry, Vol. 380, Issue 11, pp. 1307-14, 2000 (PubMed).

Fuhlendorff, Clemmensen, Magnusson: "Primary structure of tetranectin, a plasminogen kringle 4 binding plasma protein: homology with asialoglycoprotein receptors and cartilage proteoglycan core protein." in: Biochemistry, Vol. 26, Issue 21, pp. 6757-64, 1988 (PubMed).