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  • 产品名称：CD3dMolecule,delta(CD3-TCRComplex)(CD3D)(AA22-105)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD3dMolecule,delta(CD3-TCRComplex)(CD3D)(AA22-105)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.3 kDa. The protein migrates as 26-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background CD3D is also known as CD3-DELTA, T3D, and is a single-pass type I membrane protein. CD3D is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
Molecular Weight 14.3 kDa
UniProt Q95LI8
Pathways TCR Signaling, CXCR4-mediated Signaling Events
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 22-105) (Active) protein (His tag) (ABIN2180770) CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 22-105) (Active) protein (His tag)
Background publications Gil, Busto, Garcillán, Chean, García-Rodríguez, Díaz-Alderete, Navarro, Reiné, Mencía, Gurbindo, Beléndez, Gordillo, Duchniewicz, Höhne, García-Sánchez, Fernández-Cruz, López-Granados, Schamel et al.: "A leaky mutation in CD3D differentially affects ?? and ?? T cells and leads to a T??-T??+B+NK+ human SCID. ..." in: The Journal of clinical investigation, Vol. 121, Issue 10, pp. 3872-6, 2011 (PubMed).