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  • 产品名称：CD70Molecule(CD70)(AA39-193)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD70Molecule(CD70)(AA39-193)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 44.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Immobilized mouse CD27 at 2 μg/mL ( 100 μL/well ) can bind human CD70 with a linear range of 62 - 1000 pg/mL.

SDS-PAGE: due to monomeric, dimeric or trimeric form and different glycosylation.
Background Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.
Molecular Weight 44.6 kDa
UniProt P32970
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD70 Molecule (CD70) (AA 39-193) (Active) protein (Fc Tag) (ABIN2180828) Human CD27 Ligand, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stain...
Binding Studies (Bind) image for CD70 Molecule (CD70) (AA 39-193) (Active) protein (Fc Tag) (ABIN2180828) Immobilized Human CD27 Ligand, Fc Tag with a linear range of 4.9-156 ng/mL.
Background publications Raman, Akondy, Rath, Bal, George: "Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 11, pp. 5876-81, 2003 (PubMed).

Kobata, Jacquot, Kozlowski, Agematsu, Schlossman, Morimoto: "CD27-CD70 interactions regulate B-cell activation by T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 24, pp. 11249-53, 1995 (PubMed).