产品详情
查看大图

  • 产品名称：Kallikrein-RelatedPeptidase4(KLK4)(AA27-254)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Kallikrein-RelatedPeptidase4(KLK4)(AA27-254)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.2 kDa. The protein migrates as 30-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. (R&D Systems, Catalog # ES011) The specific activity is >200 pmoles / min / μg.
Background Kallikrein-4 (KLK4) is also known as Enamel matrix serine proteinase 1 (EMSP1), Kallikrein-like protein 1 (KLK-L1), Prostase, Serine protease 17 (PRSS17), PSTS, which belongs to the peptidase S1 family and Kallikrein subfamily. KLK4 contains one peptidase S1 domain. KLK4 is expressed in prostate and involved in enamel formation. Defects in Kallikrein-4 / KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions.
Molecular Weight 25.2 kDa
UniProt Q9Y5K2
Pathways Complement System
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Kallikrein-Related Peptidase 4 (KLK4) (AA 27-254) (Active) protein (His tag) (ABIN2181438) Human Kallikrein 4, His Tag on SDS-PAGE under reducing (R) condition. The gel was sta...
Background publications Carleton, Collimore, Asmundson: "Social anxiety and fear of negative evaluation: construct validity of the BFNE-II." in: Journal of anxiety disorders, Vol. 21, Issue 1, pp. 131-41, 2007 (PubMed).

Hart, Hart, Michalec, Ryu, Simmons, Hong, Wright: "Mutation in kallikrein 4 causes autosomal recessive hypomaturation amelogenesis imperfecta." in: Journal of medical genetics, Vol. 41, Issue 7, pp. 545-9, 2004 (PubMed).

Hu, Zhang, Sun, Yang, Cao, Ryu, Simmer: "Characterization of the mouse and human PRSS17 genes, their relationship to other serine proteases, and the expression of PRSS17 in developing mouse incisors." in: Gene, Vol. 251, Issue 1, pp. 1-8, 2000 (PubMed).

Hays, Chen, Hu: "Two-hybrid system for characterization of protein-protein interactions in E. coli." in: BioTechniques, Vol. 29, Issue 2, pp. 288-90, 292, 294 passim, 2000 (PubMed).