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  • 产品名称：CarnosineDipeptidase1(MetallopeptidaseM20Family)(CNDP1)(AA28-508)(Active)protei

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  • 产品厂商：ACROBiosystems
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简单介绍：
CarnosineDipeptidase1(MetallopeptidaseM20Family)(CNDP1)(AA28-508)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 54.7 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave carnosine ( beta -Ala-L-His) in a two-step assay. The specific activity is >250 pmol/min/μg.
Background Beta-Ala-His dipeptidase is also known as Carnosine dipeptidase 1 (CNDP1), CNDP dipeptidase 1, Glutamate carboxypeptidase-like protein 2, Serum carnosinase, CPGL2, which belongs to the peptidase M20A family. CNDP1 is found in serum and ***** nervous central system. CNDP1 preferential hydrolysis of the beta-Ala-|-His dipeptide (carnosine), and also anserine, Xaa-|-His dipeptides and other dipeptides including homocarnosine. The catalytic activity of CNDP1 is inhibited by the metal chelator 1,10-o-phenantrolin and the inhibitory concentration 50 % (IC50) is 5 μM.
Molecular Weight 54.7 kDa
UniProt Q96KN2
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Carnosine Dipeptidase 1 (Metallopeptidase M20 Family) (CNDP1) (AA 28-508) (Active) protein (His tag) (ABIN2180890) Human CNDP1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Braune, von Eisenhart-Rothe, Welsch, Teufel, Jaeger: "Mid-term results and quantitative comparison of postoperative shoulder function in traumatic and non-traumatic rotator cuff tears." in: Archives of orthopaedic and trauma surgery, Vol. 123, Issue 8, pp. 419-24, 2003 (PubMed).

Teufel, Saudek, Ledig, Bernhardt, Boularand, Carreau, Cairns, Carter, Cowley, Duverger, Ganzhorn, Guenet, Heintzelmann, Laucher, Sauvage, Smirnova: "Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase." in: The Journal of biological chemistry, Vol. 278, Issue 8, pp. 6521-31, 2003 (PubMed).

Lenney, Peppers, Kucera, Sjaastad: "Homocarnosinosis: lack of serum carnosinase is the defect probably responsible for elevated brain and CSF homocarnosine." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 132, Issue 2, pp. 157-65, 1983 (PubMed).

Lenney, George, Weiss, Kucera, Chan, Rinzler: "Human serum carnosinase: characterization, distinction from cellular carnosinase, and activation by cadmium." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 123, Issue 3, pp. 221-31, 1982 (PubMed).