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  • 产品名称：CystatinSA(CST2)(AA21-141)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CystatinSA(CST2)(AA21-141)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.2 kDa. The protein migrates as 15-17 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value is<10.0 nm.="" <="" td="">
Background Cystatin-2 (CST2) is also known as Cystatin-SA, Cystatin-S5, is a secreted thiol protease inhibitor, which belongs to the cystatin family. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The CST1, CST2, CST4, and CST5 are expressed in differential, tissue-specific patterns. CST2 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) and is also expressed in submandibular gland and parotid gland.
Molecular Weight 15.2 kDa
UniProt P09228
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 25 mM Tris, 150 mM NaCl, pH 8.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cystatin SA (CST2) (AA 21-141) (Active) protein (His tag) (ABIN2180918) Human Cystatin SA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications Ryan, Souda, Halgand, Wong, Loo, Faull, Whitelegge: "Confident assignment of intact mass tags to human salivary cystatins using top-down Fourier-transform ion cyclotron resonance mass spectrometry." in: Journal of the American Society for Mass Spectrometry, Vol. 21, Issue 6, pp. 908-17, 2010 (PubMed).

Dickinson, Thiesse, Hicks: "Expression of type 2 cystatin genes CST1-CST5 in ***** human tissues and the developing submandibular gland." in: DNA and cell biology, Vol. 21, Issue 1, pp. 47-65, 2002 (PubMed).