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  • 产品名称：serpinPeptidaseInhibitor,CladeA(Alpha-1Antiproteinase,Antitrypsin),Member1(SERP

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  • 产品厂商：ACROBiosystems
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简单介绍：
serpinPeptidaseInhibitor,CladeA(Alpha-1Antiproteinase,Antitrypsin),Member1(SERPINA1)(AA25-418)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 45.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog #T1426 ), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl , pH 7.5. The IC50 value is approximately <4.5 nm.="" <="" td="">
Background Serpin A1 is also known as Alpha-1-antitrypsin (A1AT), serum trypsin inhibitor, alpha-1 proteinase inhibitor (A1PI), AAT, which belongs to the serpin family. Most serpins inactivate enzymes by binding to them covalently, requiring very high levels to perform their function. Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Serpin A1 / A1AT is inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form of SerpinA1 inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Serpin A1 / A1AT protects tissues from enzymes of inflammatory cells, especially neutrophil elastase. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD).
Molecular Weight 45.1 kDa
UniProt P01009
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) (AA 25-418) (Active) protein (His tag) (ABIN2181744) Human Serpin A1, His Tag on SDS-PAGE under reducing (R) condition. The gel was staine...
Background publications Niemann, Narkates, Miller: "Isolation and serine protease inhibitory activity of the 44-residue, C-terminal fragment of alpha 1-antitrypsin from human placenta." in: Matrix (Stuttgart, Germany), Vol. 12, Issue 3, pp. 233-41, 1992 (PubMed).

Tanaka, Sekiya, Takamizawa, Kato, Moriyama, Fujimura: "Characterization of a 54 kDa, alpha 1-antitrypsin-like protein isolated from ascitic fluid of an endometrial cancer patient." in: Japanese journal of cancer research : Gann, Vol. 82, Issue 6, pp. 693-700, 1991 (PubMed).