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  • 产品名称：ProgrammedCellDeath1Ligand2(PDCD1LG2)(AA20-219)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ProgrammedCellDeath1Ligand2(PDCD1LG2)(AA20-219)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.4 kDa. The protein migrates as 35-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rh B7-DC / PD-L2 / CD273 at 1 μg/mL (100 μL/well) can bind rhPDCD-1 with a linear range of 15 - 500 ng/mL.
Background Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Molecular Weight 23.4 kDa
UniProt Q9BQ51
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 20-219) (Active) protein (His tag) (ABIN2181599) Human PD-L2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Binding Studies (Bind) image for Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 20-219) (Active) protein (His tag) (ABIN2181599) Immobilized Human PD-L2, His Tag with a linear range of 0.02-0.5 μg/mL.
Background publications He, Liu, Xu, Zeng: "Cloning and identification of two novel splice variants of human PD-L2." in: Acta biochimica et biophysica Sinica, Vol. 36, Issue 4, pp. 284-9, 2004 (PubMed).

Tseng, Otsuji, Gorski, Huang, Slansky, Pai, Shalabi, Shin, Pardoll, Tsuchiya: "B7-DC, a new dendritic cell molecule with potent costimulatory properties for T cells." in: The Journal of experimental medicine, Vol. 193, Issue 7, pp. 839-46, 2001 (PubMed).

Latchman, Wood, Chernova, Chaudhary, Borde, Chernova, Iwai, Long, Brown, Nunes, Greenfield, Bourque, Boussiotis, Carter, Carreno, Malenkovich, Nishimura, Okazaki, Honjo, Sharpe, Freeman: "PD-L2 is a second ligand for PD-1 and inhibits T cell activation." in: Nature immunology, Vol. 2, Issue 3, pp. 261-8, 2001 (PubMed).