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  • 产品名称：Osteoactivin(AA22-486)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Osteoactivin(AA22-486)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 52.9 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by the ability of the immobilized protein to induce pro-MMP-9 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 4 - 16 μg/mL.
Background Transmembrane glycoprotein NMB (GPNMB) is also known as Transmembrane glycoprotein HGFIN, DC-HIL and Osteoactivin (OA), which belongs to the PMEL/NMB family. GPNMB contains one PKD domain. GPNMB is a transmembrane glycoprotein that is up-regulated in various cancer cells, including in glioblastoma multiforme and is expressed in many melanoma cells, as well as in tissue macrophages. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages.
Molecular Weight 52.9 kDa
UniProt Q14956
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Osteoactivin (AA 22-486) (Active) protein (His tag) (ABIN2181188) Human Osteoactivin, His Tag on SDS-PAGE under reducing (R) condition. The gel was sta...
Background publications Abdelmagid, Barbe, Rico, Salihoglu, Arango-Hisijara, Selim, Anderson, Owen, Popoff, Safadi: "Osteoactivin, an anabolic factor that regulates osteoblast differentiation and function." in: Experimental cell research, Vol. 314, Issue 13, pp. 2334-51, 2008 (PubMed).