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  • 产品名称：Sparc/osteonectin,CwcvandKazal-LikeDomainsProteoglycan(Testican)1(SPOCK1)(AA22-

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  • 产品厂商：ACROBiosystems
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简单介绍：
Sparc/osteonectin,CwcvandKazal-LikeDomainsProteoglycan(Testican)1(SPOCK1)(AA22-439)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 47.8 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >85 % as determined by reduced SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC.

SDS-PAGE: due to glycosylation.
Background Testican-1 is also known as protein SPOCK,SPOCK1, which contains one azal-like domain and one thyroglobulin type-1 domain.SPOCK1 is an extracellular heparan/chondroitin sulfate proteoglycan. Members of this family are known as testicans, also called SPOCKs. SPOCKs are enriched in brain and have been shown to regulate neuronal attachment and outgrowth. They contain inhibitory regions in several domains targeted to different classes of protease, and in some cases may act as protease inhibitors. Little is known about SPOCK1's function until now. It may play a role in cell-cell and cell-matrix interactions except for contributing to various neuronal mechanisms in the central nervous system.
Molecular Weight 47.8 kDa
UniProt Q08629
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 1 (SPOCK1) (AA 22-439) (Active) protein (His tag) (ABIN2181787) Human Testican 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stain...
Background publications Bonnet, Perin, Maillet, Jolles, Alliel: "Characterization of a human seminal plasma glycosaminoglycan-bearing polypeptide." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 565-9, 1993 (PubMed).

Alliel, Perin, Jollès, Bonnet: "Testican, a multidomain testicular proteoglycan resembling modulators of cell social behaviour." in: European journal of biochemistry / FEBS, Vol. 214, Issue 1, pp. 347-50, 1993 (PubMed).