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  • 产品名称：Tim-1(AA21-290)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Tim-1(AA21-290)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 29.6 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit antiCD3induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and rhTIM1. The ED50 for this effect is typically 0.15-1.0 μg/mL.

SDS-PAGE: rhHAVCR1 protein migrates as 80-100 kDa poly peptide due to high glycosylation.
Background Hepatitis A virus cellular receptor 1 is also known as HAVCR1, HAVCR, KIM1, TIM, TIM1, TIMD1, is widely expressed with highest levels in kidney and testis. The protein encoded by HAVCR1 gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. Three transcript variants encoding the same protein have been found for this gene. HAVCR1 may play a role in T-helper cell development, the regulation of asthma and allergic diseases and in kidney injury and repair. In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus.
Molecular Weight 29.6 kDa
UniProt Q96D42
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tim-1 (AA 21-290) (Active) protein (His tag) (ABIN2181417) Human TIM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Silberstein, Dveksler, Kaplan: "Neutralization of hepatitis A virus (HAV) by an immunoadhesin containing the cysteine-rich region of HAV cellular receptor-1." in: Journal of virology, Vol. 75, Issue 2, pp. 717-25, 2001 (PubMed).