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  • 产品名称：FumarateHydratase(FH)(AA45-510)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
FumarateHydratase(FH)(AA45-510)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 51 kDa. The protein migrates as 47 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Fumarate hydratase, mitochondrial is also known as Fumarase or FH, which belongs to the class-II fumarase/aspartase family and Fumarase subfamily. Fumarase is a homotetramer protein which is expressed in red blood cells. There are two substrate-s: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors. Fumarase also acts as a tumor suppressor.
Molecular Weight 51 kDa
UniProt P07954
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Fumarate Hydratase (FH) (AA 45-510) (Active) protein (His tag) (ABIN2181102) Fumarate Hydratase (FH) (AA 45-510) (Active) protein (His tag)
Background publications Choudhary, Olsen, Brandts, Cox, Reddy, Böhmer, Gerke, Schmidt-Arras, Berdel, Müller-Tidow, Mann, Serve: "Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes." in: Molecular cell, Vol. 36, Issue 2, pp. 326-39, 2009 (PubMed).

Choudhary, Kumar, Gnad, Nielsen, Rehman, Walther, Olsen, Mann: "Lysine acetylation targets protein complexes and co-regulates major cellular functions." in: Science (New York, N.Y.), Vol. 325, Issue 5942, pp. 834-40, 2009 (PubMed).

Tomlinson, Alam, Rowan, Barclay, Jaeger, Kelsell, Leigh, Gorman, Lamlum, Rahman, Roylance, Olpin, Bevan, Barker, Hearle, Houlston, Kiuru, Lehtonen, Karhu, Vilkki, Laiho, Eklund, Vierimaa, Aittomäki et al.: "Germline mutations in FH predispose to dominantly inherited uterine fibroids, skin leiomyomata and papillary renal cell cancer. ..." in: Nature genetics, Vol. 30, Issue 4, pp. 406-10, 2002 (PubMed).