产品详情
  • 产品名称:FcFragmentofIgG,LowAffinityIIa,Receptor(CD32)(FCGR2A)(AA34-218)(Active)protein(

  • 产品型号:
  • 产品厂商:ACROBiosystems
  • 产品文档:
你添加了1件商品 查看购物车
简单介绍:
FcFragmentofIgG,LowAffinityIIa,Receptor(CD32)(FCGR2A)(AA34-218)(Active)protein(StrepTag,Histag)
详情介绍:
Brand MABSol®
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.2 kDa. The protein migrates as 29-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized Human CRP/ C-reactive Protein (Cat# CRP-H5226) at 5μg/mL,can bind Biotinylated Human Fcgamma RIIA /CD32a Protein with a linear range of 0.1-10 μg/mL.

SDS-PAGE: due to glycosylation.
Background Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
Molecular Weight 21.2 kDa
UniProt Q92835
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 34-218) (Active) protein (Strep Tag,His tag) (ABIN2180752) Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 34-218) (Active) protein (Strep Tag,His tag)
Background publications Takai: "Roles of Fc receptors in autoimmunity." in: Nature reviews. Immunology, Vol. 2, Issue 8, pp. 580-92, 2002 (PubMed).

Hawiger, Inaba, Dorsett, Guo, Mahnke, Rivera, Ravetch, Steinman, Nussenzweig: "Dendritic cells induce peripheral T cell unresponsiveness under steady state conditions in vivo." in: The Journal of experimental medicine, Vol. 194, Issue 6, pp. 769-79, 2001 (PubMed).