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  • 产品名称：Mannose-BindingLectinC(AA21-248)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Mannose-BindingLectinC(AA21-248)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 24.8 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind with Mannan in a functional ELISA.
Background Mannose-binding lectin (MBL) is also known as mannose-binding protein, mannan-binding protein (MBP), Mannose-binding protein C, Collectin-1 (COLEC1), MBL2, which belongs to the class of collectins in the C-type lectin superfamily. MBL contains one C-type lectin domain and one collagen-like domain. MBL has an oligomeric structure (400-700 kDa), built of subunits that contain three presumably identical peptide chains of about 30 kDa each. MBL is calcium-dependent lectin involved in innate immune defense. MBL binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. MBL binds to late apoptotic cells. MBL may bind DNA.
Molecular Weight 24.8 kDa
UniProt P11226
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Mannose-Binding Lectin C (AA 21-248) (Active) protein (His tag) (ABIN2181498) Human MBL, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained over...
Background publications Hirano, Ma, Kawasaki, Okimura, Baba, Nakagawa, Miwa, Kawasaki, Oka, Kawasaki: "Mannan-binding protein blocks the activation of metalloproteases meprin alpha and beta." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 3177-85, 2005 (PubMed).

Nauta, Raaschou-Jensen, Roos, Daha, Madsen, Borrias-Essers, Ryder, Koch, Garred: "Mannose-binding lectin engagement with late apoptotic and necrotic cells." in: European journal of immunology, Vol. 33, Issue 10, pp. 2853-63, 2003 (PubMed).