产品详情
  • 产品名称:PolymericImmunoglobulinReceptor(PIGR)(AA19-638)(Active)protein(Histag)

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  • 产品厂商:ACROBiosystems
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简单介绍:
PolymericImmunoglobulinReceptor(PIGR)(AA19-638)(Active)protein(Histag)
详情介绍:
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 68.7 kDa. The protein migrates as 85-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human PIGR at 2 μg/mL, the concentration of human IgM that produces 50% of the optimal binding response is appoximately 20-200 ng/mL.
Background Polymeric immunoglobulin receptor (PIGR) is also known as Poly-Ig receptor, which can be cleaved into membrane secretory component (SC). PIGR is a type I transmembrane glycoprotein or a member of the immunoglobulin superfamily, and is synthesized by secretory epithelial cells and delivered to the basolateral plasma membrane where it can bind larger polymers of IgA (pIgA ) and pentameric IgM. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
Molecular Weight 68.7 kDa
UniProt P01833
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Polymeric Immunoglobulin Receptor (PIGR) (AA 19-638) (Active) protein (His tag) (ABIN2181649) Human pIgR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Hamburger, West, Bjorkman: "Crystal structure of a polymeric immunoglobulin binding fragment of the human polymeric immunoglobulin receptor." in: Structure (London, England : 1993), Vol. 12, Issue 11, pp. 1925-35, 2004 (PubMed).

Hughes, Frutiger, Savoy, Reason, Morris, Jaton: "Human free secretory component is composed of the first 585 amino acid residues of the polymeric immunoglobulin receptor." in: FEBS letters, Vol. 410, Issue 2-3, pp. 443-6, 1997 (PubMed).

Krajci, Grzeschik, Geurts van Kessel, Olaisen, Brandtzaeg: "The human transmembrane secretory component (poly-Ig receptor): molecular cloning, restriction fragment length polymorphism and chromosomal sublocalization." in: Human genetics, Vol. 87, Issue 6, pp. 642-8, 1991 (PubMed).

Eiffert, Quentin, Decker, Hillemeir, Hufschmidt, Klingmüller, Weber, Hilschmann: "[The primary structure of human free secretory component and the arrangement of disulfide bonds]." in: Hoppe-Seyler's Zeitschrift für physiologische Chemie, Vol. 365, Issue 12, pp. 1489-95, 1985 (PubMed).