GrowthHormoneReceptor(GHR)(AA27-264)(Active)protein(FcTag) 抗体网

产品详情

所在位置: 首页> 产品目录> Proteins（蛋白）>

查看大图

产品名称：GrowthHormoneReceptor(GHR)(AA27-264)(Active)protein(FcTag)
产品型号：
产品厂商：ACROBiosystems
产品文档：

你添加了1件商品查看购物车

简单介绍：

GrowthHormoneReceptor(GHR)(AA27-264)(Active)protein(FcTag)

详情介绍：

Product details
Product details
Characteristics	This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 54.3 kDa. As a result of glycosylation, the protein migrates as 66-85 kDa under reducing (R) condition, and 135-160 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity	>95 % as determined by SDS-PAGE.
Sterility	0.22 μm filtered
Endotoxin Level	Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment	Biological Activity: Measured by its ability to inhibit GH-induced proliferation of Nb2-11 rat lymphoma cells in the presence of 0.2 ng/mL of rhGH. The ED50 for this effect is typically 0.8 - 3.5 ng/mL.

Target details
Target details
Background	Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
Molecular Weight	54.3 kDa
UniProt	P10912
Pathways	NF-kappaB Signaling, JAK-STAT Signaling, Response to Growth Hormone Stimulus

Application Details
Application Details
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer	50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice	Avoid repeated freeze-thaw cycles.
Storage	-20 °C
Storage Comment	No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Images
Images
Supplier Images	Human Growth Hormone R, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) co... Human Growth Hormone R, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. Human Growth Hormone R, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

References
References
Background publications	Ghizzoni, Duquesnoy, Torresani, Vottero, Goossens, Bernasconi: "Isolated growth hormone deficiency type IA associated with a 45-kilobase gene deletion within the human growth hormone gene cluster in an Italian family." in: Pediatric research, Vol. 36, Issue 5, pp. 654-9, 1995 (PubMed). Duquesnoy, Sobrier, Duriez, Dastot, Buchanan, Savage, Preece, Craescu, Blouquit, Goossens et al.: "A single amino acid substitution in the exoplasmic domain of the human growth hormone (GH) receptor confers familial GH resistance (Laron syndrome) with positive GH-binding activity by abolishing ..." in: The EMBO journal, Vol. 13, Issue 6, pp. 1386-95, 1994 (PubMed). Urbanek, Russell, Cooke, Liebhaber: "Functional characterization of the alternatively spliced, placental human growth hormone receptor." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 19025-32, 1993 (PubMed).