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  • 产品名称：Interleukin7Receptor(IL7R)(AA21-236)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Interleukin7Receptor(IL7R)(AA21-236)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51 kDa. The protein migrates as 66-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rhIL7Ra Fc Chimera at 0.25 μg/mL ( 100 μL/well ) on a Rabbit anti-human IgG Fc antibody, the concentration of rhIL-7 that produces 50% of the optimal binding response is found to be approximately 20 - 100 ng/mL.
Background Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra /CD127 contains 1 fibronectin type-III domain. IL7Ra /CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra /CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.
Molecular Weight 51 kDa
NCBI Accession NP_002176
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Interleukin 7 Receptor (IL7R) (AA 21-236) (Active) protein (Fc Tag) (ABIN2181401) Human IL-7 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications McElroy, Dohm, Walsh: "Structural and biophysical studies of the human IL-7/IL-7Ralpha complex." in: Structure (London, England : 1993), Vol. 17, Issue 1, pp. 54-65, 2009 (PubMed).