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  • 产品名称：Interleukin27Receptor,alpha(IL27RA)(AA33-514)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Interleukin27Receptor,alpha(IL27RA)(AA33-514)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 79 kDa. The protein migrates as 105-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human IL27 at 20 μg/mL ( 100 μL/well ) can bind rh IL27RA / WSX-1 / TCCR Fc Chimera with a linear ranger of 0.064 - 1.6 μg/mL.
Background Interleukin-27 receptor subunit alpha ( IL27RA) is also known as Cytokine receptor WSX-1, Cytokine receptor-like 1, Type I T-cell cytokine receptor (TCCR), ZcytoR1, IL27RA belongs to the type I cytokine receptor family and type 2 subfamily. IL27RA contains three fibronectin type-III domains. IL27RA is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. IL27RA is receptor for IL27, but requires IL6ST/gp130 to mediate signal transduction in response to IL27. IL27RA involved in the regulation of Th1-type immune responses. IL27RA also appears to be involved in innate defense mechanisms.
Molecular Weight 79 kDa
UniProt Q6UWB1
Pathways Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Interleukin 27 Receptor, alpha (IL27RA) (AA 33-514) (Active) protein (Fc Tag) (ABIN2181379) Human IL-27 Ra, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained ...
Background publications Larousserie, Pflanz, Coulomb-LHerminé, Brousse, Kastelein, Devergne: "Expression of IL-27 in human Th1-associated granulomatous diseases." in: The Journal of pathology, Vol. 202, Issue 2, pp. 164-71, 2004 (PubMed).

Pflanz, Hibbert, Mattson, Rosales, Vaisberg, Bazan, Phillips, McClanahan, de Waal Malefyt, Kastelein: "WSX-1 and glycoprotein 130 constitute a signal-transducing receptor for IL-27." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 4, pp. 2225-31, 2004 (PubMed).

Chen, Ghilardi, Wang, Baker, Xie, Gurney, Grewal, de Sauvage: "Development of Th1-type immune responses requires the type I cytokine receptor TCCR." in: Nature, Vol. 407, Issue 6806, pp. 916-20, 2000 (PubMed).

Sprecher, Grant, Baumgartner, Presnell, Schrader, Yamagiwa, Whitmore, OHara, Foster: "Cloning and characterization of a novel class I cytokine receptor." in: Biochemical and biophysical research communications, Vol. 246, Issue 1, pp. 82-90, 1998 (PubMed).