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  • 产品名称：RegeneratingIslet-DerivedFamily,Member4(REG4)(AA23-158)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
RegeneratingIslet-DerivedFamily,Member4(REG4)(AA23-158)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.7 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by the ability of the immobilized protein to support the proliferation of HCT-116 human colorectal carcinoma cells (ATCC: CCL-247 ) under low serum conditions. The ED50 for this effect is typically 3-15 μg/mL.
Background Regenerating islet-derived protein 4 (REG4) is also known as Gastrointestinal secretory protein (GISP), REG-like protein (RELP). REG4 contains one C-type lectin domain. REG4 has calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. REG4 may be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
Molecular Weight 16.7 kDa
UniProt Q9BYZ8
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Regenerating Islet-Derived Family, Member 4 (REG4) (AA 23-158) (Active) protein (His tag) (ABIN2181689) Human Reg4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Ho, Lou, Wei, Luo, Lin, Lyu, Chen: "Human RegIV protein adopts a typical C-type lectin fold but binds mannan with two calcium-independent sites." in: Journal of molecular biology, Vol. 402, Issue 4, pp. 682-95, 2010 (PubMed).

Kämäräinen, Heiskala, Knuutila, Heiskala, Winqvist, Andersson: "RELP, a novel human REG-like protein with up-regulated expression in inflammatory and metaplastic gastrointestinal mucosa." in: The American journal of pathology, Vol. 163, Issue 1, pp. 11-20, 2003 (PubMed).