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  • 产品名称：CD274Molecule(CD274)(AA19-238)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD274Molecule(CD274)(AA19-238)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.1 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
Molecular Weight 27.1 kDa
UniProt G7PSE7
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD274 Molecule (CD274) (AA 19-238) (Active) protein (His tag) (ABIN2181633) Cynomolgus PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stain...
Background publications Freeman, Long, Iwai, Bourque, Chernova, Nishimura, Fitz, Malenkovich, Okazaki, Byrne, Horton, Fouser, Carter, Ling, Bowman, Carreno, Collins, Wood, Honjo: "Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation." in: The Journal of experimental medicine, Vol. 192, Issue 7, pp. 1027-34, 2000 (PubMed).

Dong, Zhu, Tamada, Chen: "B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion." in: Nature medicine, Vol. 5, Issue 12, pp. 1365-9, 1999 (PubMed).