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  • 产品名称：Neuropilin1(NRP1)(AA22-644)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Neuropilin1(NRP1)(AA22-644)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.7 kDa. The protein migrates as 85-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind with recombinant human VEGF165 in a functional ELISA.

SDS-PAGE: due to glycosylation.
Background Neuropilin-1 (NRP1) is also known as Vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells.
Molecular Weight 70.7 kDa
UniProt O14786
Pathways Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Neuropilin 1 (NRP1) (AA 22-644) (Active) protein (His tag) (ABIN2181552) Human Neuropilin-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was sta...
Binding Studies (Bind) image for Neuropilin 1 (NRP1) (AA 22-644) (Active) protein (His tag) (ABIN2181552) Immobilized Human Neuropilin-1, His Tag (Cat # NR1-H5228) at 2 μg/mL (100 μL/well) ca...
Background publications Appleton, Wu, Maloney, Yin, Liang, Stawicki, Mortara, Bowman, Elliott, Desmarais, Bazan, Bagri, Tessier-Lavigne, Koch, Wu, Watts, Wiesmann: "Structural studies of neuropilin/antibody complexes provide insights into semaphorin and VEGF binding." in: The EMBO journal, Vol. 26, Issue 23, pp. 4902-12, 2007 (PubMed).

Tamagnone, Artigiani, Chen, He, Ming, Song, Chedotal, Winberg, Goodman, Poo, Tessier-Lavigne, Comoglio: "Plexins are a large family of receptors for transmembrane, secreted, and GPI-anchored semaphorins in vertebrates." in: Cell, Vol. 99, Issue 1, pp. 71-80, 1999 (PubMed).