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  • 产品名称：beta-1,3-Glucuronyltransferase3(GlucuronosyltransferaseI)(B3GAT3)(AA72-335)(Act

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  • 产品厂商：ACROBiosystems
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简单介绍：
beta-1,3-Glucuronyltransferase3(GlucuronosyltransferaseI)(B3GAT3)(AA72-335)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 30.2 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE).
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3), a member of the glycosyltransferase 43 family, is also known as beta-1,3-glucuronyltransferase 3, glucuronosyltransferase I (GlcAT-I) and UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase (GlcUAT-I). B3GAT3 plays a role in glycosaminoglycans biosynthesis and be involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. B3GAT3 can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Furthermore,B3GAT3 shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc.
Molecular Weight 30.2 kDa
UniProt O94766
Pathways Glycosaminoglycan Metabolic Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 8.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 72-335) (Active) protein (His tag) (ABIN2180616) Human B3GAT3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained o...
Background publications Tone, Pedersen, Yamamoto, Izumikawa, Kitagawa, Nishihara, Tamura, Negishi, Sugahara et al.: "2-o-phosphorylation of xylose and 6-o-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage region ..." in: The Journal of biological chemistry, Vol. 283, Issue 24, pp. 16801-7, 2008 (PubMed).