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  • 产品名称：V-Erb-B2erythroblasticLeukemiaViralOncogeneHomolog2,Neuro/glioblastomaDerivedOn

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  • 产品厂商：ACROBiosystems
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简单介绍：
V-Erb-B2erythroblasticLeukemiaViralOncogeneHomolog2,Neuro/glioblastomaDerivedOncogeneHomolog(Avian)(ERBB2)(AA23-652)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 97.7 kDa. The protein migrates as 130-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer cell proliferation. The ED50 for this effect is typically 20-80 ng/mL in the presence of 0.6 μg/mL goat anti-hErbB2. Validated Herceptin Mab binding in a functional ELISA.
Background Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30 % of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation .
Molecular Weight 97.7 kDa
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) (Active) protein (Fc Tag) (ABIN2181216) Human Her2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained over...
Binding Studies (Bind) image for V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) (Active) protein (Fc Tag) (ABIN2181216) Immobilized Human Her2, Fc Tag (Cat# HE2-H5253) at 2 μg/mL (100 μl/well) can bind tra...
Product cited in: Piechocki, Wu, Jones, Jacob, Gibson, Ethier, Abrams, Yagita, Venuprasad, Wei: "Induction of proapoptotic antibodies to triple-negative breast cancer by vaccination with TRAIL death receptor DR5 DNA." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 11, pp. 2562-72, 2012 (PubMed).

Background publications Codony-Servat, Albanell, Lopez-Talavera, Arribas, Baselga: "Cleavage of the HER2 ectodomain is a pervanadate-activable process that is inhibited by the tissue inhibitor of metalloproteases-1 in breast cancer cells." in: Cancer research, Vol. 59, Issue 6, pp. 1196-201, 1999 (PubMed).

Carraway, Rossi, Komatsu, Price-Schiavi, Huang, Guy, Carvajal, Fregien, Carraway, Carraway: "An intramembrane modulator of the ErbB2 receptor tyrosine kinase that potentiates neuregulin signaling." in: The Journal of biological chemistry, Vol. 274, Issue 9, pp. 5263-6, 1999 (PubMed).

Tzahar, Yarden: "The ErbB-2/HER2 oncogenic receptor of adenocarcinomas: from orphanhood to multiple stromal ligands." in: Biochimica et biophysica acta, Vol. 1377, Issue 1, pp. M25-37, 1998 (PubMed).