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  • 产品名称：ThiaminPyrophosphokinase1(TPK1)(AA2-243)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ThiaminPyrophosphokinase1(TPK1)(AA2-243)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 28 kDa. The protein migrates as 28 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Thiamin pyrophosphokinase 1 (TPK1),a member of the thiamine pyrophosphokinase family, is also known as Thiamine pyrophosphokinase 1, Placental protein 20 (PP20) and hTPK1,which is detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.TPK1 is regarded as a Kinase and a Transferase that can bind ATP and Nucleotide. TPK1 catalyzes the phosphorylation of thiamine to thiamine pyrophosphate and can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. As for disease,TPK1 is relevant to thiamine metabolism dysfunction syndrome 5, episodic encephalopathy type (THMD5).
Molecular Weight 28 kDa
UniProt Q9H3S4
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 8.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Thiamin Pyrophosphokinase 1 (TPK1) (AA 2-243) (Active) protein (His tag) (ABIN2181858) Human TPK1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Mayr, Freisinger, Schlachter, Rolinski, Zimmermann, Scheffner, Haack, Koch, Ahting, Prokisch, Sperl: "Thiamine pyrophosphokinase deficiency in encephalopathic children with defects in the pyruvate oxidation pathway." in: American journal of human genetics, Vol. 89, Issue 6, pp. 806-12, 2011 (PubMed).

Bellyei, Szigeti, Boronkai, Szabo, Bene, Janaky, Barna, Sipos, Minik, Kravjak, Ohmacht, Melegh, Zavodszky, Than, Sumegi, Bohn, Than: "Cloning, sequencing, structural and molecular biological characterization of placental protein 20 (PP20)/human thiamin pyrophosphokinase (hTPK)." in: Placenta, Vol. 26, Issue 1, pp. 34-46, 2005 (PubMed).