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  • 产品名称：UDP-Galactose-4-Epimerase(GALE)(AA1-348)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
UDP-Galactose-4-Epimerase(GALE)(AA1-348)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 39 kDa. The protein migrates as 39 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background UDP-glucose 4-epimerase, a member of the NAD(P)-dependent epimerase (dehydratase) family, is also known as galactowaldenase (GALE), UDP-N-acetylgalactosamine 4-epimerase (UDP-GalNAc 4-epimerase) and UDP-N-acetylglucosamine 4-epimerase (UDP-GlcNAc 4-epimerase),which is a homodimeric epimerase found in bacterial, fungal, plant, and mammalian cells. GALE can catalyze two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and GALE (UDP-GalNAc) when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.
Molecular Weight 39 kDa
UniProt Q14376
Pathways Response to Water Deprivation, Cellular Glucan Metabolic Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 20 mM HEPES, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for UDP-Galactose-4-Epimerase (GALE) (AA 1-348) (Active) protein (His tag) (ABIN2181138) UDP-Galactose-4-Epimerase (GALE) (AA 1-348) (Active) protein (His tag)
Background publications Schulz, Watson, Sanders, Ross, Thoden, Holden, Fridovich-Keil: "Determinants of function and substrate specificity in human UDP-galactose 4'-epimerase." in: The Journal of biological chemistry, Vol. 279, Issue 31, pp. 32796-803, 2004 (PubMed).

Thoden, Wohlers, Fridovich-Keil, Holden: "Human UDP-galactose 4-epimerase. Accommodation of UDP-N-acetylglucosamine within the active site." in: The Journal of biological chemistry, Vol. 276, Issue 18, pp. 15131-6, 2001 (PubMed).