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  • 产品名称：CytotoxicT-Lymphocyte-AssociatedProtein4(CTLA4)(AA37-160)(Active)protein(AVItag

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  • 产品厂商：ACROBiosystems
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简单介绍：
CytotoxicT-Lymphocyte-AssociatedProtein4(CTLA4)(AA37-160)(Active)protein(AVItag,Biotin,Histag)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries a Avi tag (AvitagTM) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 15.9 kDa. The protein migrates as 23-26 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rhB7-1/CD80 Protein Fc Chimera (Catalog # B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CTLA4, His.Avi Tag (Catalog # CT4-C82E5) with a linear range of 0.5 - 20 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized rhB7-2 / CD86 Fc Chimera (Catalog # CD6-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CTLA4, His.Avi Tag (Catalog # CT4-C82E5) with a linear range of 0.5 - 20 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized rhB7-1/CD80 Protein (Catalog # B71-H5228) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CTLA4, His.Avi Tag (Catalog # CT4-C82E5) with a linear range of 0.1-1 μg/mL.

SDS-PAGE: due to glycosylation. By co-expression with biotin ligase (BirA enzyme), Biotinylated Cynomolgus CTLA4, His.Avi Tag was biotinylated in vivo for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
Background CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 Molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
Molecular Weight 15.9 kDa
UniProt G7PL88
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 37-160) (Active) protein (AVI tag,Biotin,His tag) (ABIN2180929) Biotinylated Cynomolgus CTLA-4 on SDS-PAGE under reducing (R) condition. The gel was ...
Background publications Magistrelli, Jeannin, Herbault, Benoit De Coignac, Gauchat, Bonnefoy, Delneste: "A soluble form of CTLA-4 generated by alternative splicing is expressed by nonstimulated human T cells." in: European journal of immunology, Vol. 29, Issue 11, pp. 3596-602, 1999 (PubMed).