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  • 产品名称：Contactin2(Axonal)(CNTN2)(AA31-1012)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Contactin2(Axonal)(CNTN2)(AA31-1012)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 109 kDa. The protein migrates as 140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been precoated with 50 μL/well of the rhContactin2 solution at 8-30 μg/mL.

SDS-PAGE: resulting from glycosylation.
Background Contactin-2 is also known as CNTN2, TAX1 (transiently-expressed axonal glycoprotein), TAG1 (transient axonal glycoprotein), and axonin-1, and is a member of the immunoglobulin superfamily. CNTN2 consists of six Ig-like domains and four fibronectin type III domains, and is anchored to the membrane by glycosylphosphatidylinositol (GPI), whereas the soluble form can be released by a GPI-specific phospholipase. As a neural cell adhesion molecule expressed by a subset of neuronal populations in the developing CNS and PNS, CNTN2 mediates cell-cell interactions either via homophilic, or heterophilic contacts with various adhesion molecules including NgCAM, NrCAM, NCAM and neurocan. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. It may also be involved in glial tumorigenesis and may provide a potential target for therapeutic intervention.
Molecular Weight 109 kDa
NCBI Accession NP_005067
UniProt Q02246
Pathways Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: 4-8°C for 1 year in lyophilized state 4-8°C for 1 month under sterile conditions after reconstitution -20°C to -70°C for 3 months under sterile conditions after reconstitution
Supplier Images
SDS-PAGE (SDS) image for Contactin 2 (Axonal) (CNTN2) (AA 31-1012) (Active) protein (His tag) (ABIN2180893) Human Contactin-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications Traka, Goutebroze, Denisenko, Bessa, Nifli, Havaki, Iwakura, Fukamauchi, Watanabe, Soliven, Girault, Karagogeos: "Association of TAG-1 with Caspr2 is essential for the molecular organization of juxtaparanodal regions of myelinated fibers." in: The Journal of cell biology, Vol. 162, Issue 6, pp. 1161-72, 2003 (PubMed).

Tsiotra, Karagogeos, Theodorakis, Michaelidis, Modi, Furley, Jessell, Papamatheakis: "Isolation of the cDNA and chromosomal localization of the gene (TAX1) encoding the human axonal glycoprotein TAG-1." in: Genomics, Vol. 18, Issue 3, pp. 562-7, 1994 (PubMed).