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  • 产品名称：V-Erb-B2ErythroblasticLeukemiaViralOncogeneHomolog3(Avian)(ERBB3)(AA20-643)(Act

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  • 产品厂商：ACROBiosystems
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简单介绍：
V-Erb-B2ErythroblasticLeukemiaViralOncogeneHomolog3(Avian)(ERBB3)(AA20-643)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 95.4 kDa. The protein migrates as 130-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit the biological activity of Neuregulin1-β1 on MCF-7 human breast cancer cells. The ED50 for this effect is typically 1.5-6.0 μg/mL in the presence of 10 ng/mL Recombinant Human NRG1-β1/HRG1-β1 Extracellular Domain.

SDS-PAGE: resulting from glycosylation.
Background ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
Molecular Weight 95.4 kDa
NCBI Accession NP_001973
UniProt P21860
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) (AA 20-643) (Active) protein (Fc Tag) (ABIN2181037) Human ErbB3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Singer, Landgraf, Horan, Slamon, Eisenberg: "Identification of a heregulin in HER3 extracellular domain." in: The Journal of biological chemistry, Vol. 276, Issue 47, pp. 44266-74, 2001 (PubMed).

Alimandi, Romano, Curia, Muraro, Fedi, Aaronson, Di Fiore, Kraus: "Cooperative signaling of ErbB3 and ErbB2 in neoplastic transformation and human mammary carcinomas." in: Oncogene, Vol. 10, Issue 9, pp. 1813-21, 1995 (PubMed).

Gamett, Greene, Wagreich, Kim, Koland, Cerione: "Heregulin-stimulated signaling in rat pheochromocytoma cells. Evidence for ErbB3 interactions with Neu/ErbB2 and p85." in: The Journal of biological chemistry, Vol. 270, Issue 32, pp. 19022-7, 1995 (PubMed).

Carraway, Sliwkowski, Akita, Platko, Guy, Nuijens, Diamonti, Vandlen, Cantley, Cerione: "The erbB3 gene product is a receptor for heregulin." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 14303-6, 1994 (PubMed).