产品详情
查看大图

  • 产品名称：ProstateSpecificAntigen(PSA)(AA18-261)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
ProstateSpecificAntigen(PSA)(AA18-261)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.7 kDa. The protein migrates as 36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl - Arg - Pro - Tyr - p - Nitroanilide (Suc-RPY-pNA). The specific activity is >60 pmoles / min / μg.
Background Kallikrein-3 (KLK3) is also known as Prostate-specific antigen (PSA), APS, Gamma-seminoprotein (Seminin), P-30 antigen, Semenogelase, is a glycoprotein enzyme, is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. The preferential cleavage site of KLK3 is ""-Tyr-|-Xaa-"", the catalytic activity is inhibited by SERPINA5 and is strongly inhibited by Zn2+. KLK3 hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Molecular Weight 27.7 kDa
NCBI Accession NP_001639
UniProt P07288
Pathways Complement System
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Prostate Specific Antigen (PSA) (AA 18-261) (Active) protein (His tag) (ABIN2181436) Human Kallikrein 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was sta...
Background publications Pawlak, Mackessy, Sixberry, Stura, Le Du, Ménez, Foo, Ménez, Nirthanan, Kini: "Irditoxin, a novel covalently linked heterodimeric three-finger toxin with high taxon-specific neurotoxicity." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 2, pp. 534-45, 2009 (PubMed).

Ménez, Michel, Muller, Bossus, Ducancel, Jolivet-Reynaud, Stura: "Crystal structure of a ternary complex between human prostate-specific antigen, its substrate acyl intermediate and an activating antibody." in: Journal of molecular biology, Vol. 376, Issue 4, pp. 1021-33, 2008 (PubMed).

España, Gilabert, Estellés, Romeu, Aznar, Cabo et al.: "Functionally active protein C inhibitor/plasminogen activator inhibitor-3 (PCI/PAI-3) is secreted in seminal vesicles, occurs at high concentrations in human seminal plasma and complexes with ..." in: Thrombosis research, Vol. 64, Issue 3, pp. 309-20, 1992 (PubMed).